Hemopoietic Colonies

Inhibition of Hemopoietic Colonies

A possible role for natural killer (NK) ~ cells in regulating hematopoiesis was originally suggested by Cudkowicz and Hochman (1) and Kiessling et al. (2), in the mouse system. These authors showed that parental hematopoietic or lymphoid grafts do not survive in lethally irradiated F1 hybrids, even though these animals are universal recipients of grafts of other types of parental tissues. Exper...

Pluripotential stem cell differentiation in hemopoietic colonies.

To determine if mononuclear cells proliferating in murine hemopoietic spleen colonies were pluripotential in addition to possessing kinetic features of stem cells, we performed sequential studies of mice during their recovery from a split-dose irradiation regimen of 850 roentgens leg shielded-3-hr interval-850 roentgens leg irradiated (850R L.S. 3- L.I.). Injecting tritiated thymidine during st...

Studies on the regulation of hemopoietic spleen colonies.

Identification of T lymphocytes in human mixed hemopoietic colonies.

The addition of a T-cell growth-promoting medium (PHA-TCM) to culture conditions that support growth of multi-lineage hemopoietic colonies enhances the proliferation of cells with lymphoid morphology within these colonies. These cells were identified as T lymphocytes by their ability to form rosettes with SRBC and their reaction with monoclonal antibodies (OKT3, OKT4) directed against T-cell-sp...

Erythroblastic differentiation of stem cells in hemopoietic colonies.

An early phase of cellular replication precedes the initiation of erythropoiesis in hemopoietic spleen colonies of mice receiving a supralethal split-dose of irradiation, first with the leg shielded and then followed by leg irradiation 3 hr later. Previous studies indicated that this proliferative phase represents the repopulation of a depleted, endogenous stem cell compartment. To label replic...